Journal: Biomolecules

Article Title: 1-(Arylsulfonyl-isoindol-2-yl)piperazines as 5-HT 6 R Antagonists: Mechanochemical Synthesis, In Vitro Pharmacological Properties and Glioprotective Activity

doi: 10.3390/biom13010012

Figure Lengend Snippet: ( A ): Functional dose-response curve of inhibition of cAMP production at 5-HT 6 R for selected compounds 3e , 3f , and 3g in 1321N1 cells. Data were obtained from three independent experiments run in triplicate. ( B ): Impact of compounds 3e , 3f , and 3g and SB-258585 on basal cAMP production in NG108-15 cells transiently expressing 5-HT 6 R. For each compound, six independent transfection experiments were performed, and data were measured in triplicate. Data are given as means ± SEM of the values.

Article Snippet: The ability of compounds 3e , 3f , and 3g to inhibit 5-CT-induced production of cAMP was assessed using 1321N1 cells expressing the human 5-HT 6 R (PerkinElmer) using previously described procedures [ , ].

Techniques: Functional Assay, Inhibition, Expressing, Transfection

Journal: bioRxiv

Article Title: TNF- α disrupts the malate-aspartate shuttle, driving metabolic rewiring in iPSC-derived enteric neural lineages from Parkinson’s Disease patients

doi: 10.1101/2025.03.25.644826

Figure Lengend Snippet: (A) Flow cytometry characterization of iPSC purity by staining of NANOG + /LIN28A + cells. Analysis was performed before starting the initial timepoint of differentiation (day 0). n=3 biological replicates per group, mean ± SEM. (B) Statistical quantification of the gene expression of neuronal marker TUBB3 , glial marker GFAP and enteric neuronal markers PHOX2B, ELAVL4 and HOXB3 in iPSC-derived ENLs at days 6, 40 and 70 of differentiation analyzed using RT-qPCR. Log2 fold change was calculated in relation to the Iso group at day 6 of differentiation. n=3 biological replicates per group, mean ± SEM, **p<0.01, ***p<0.001, ****p<0.0001, by two-way ANOVA with Tukey’s post-hoc. (C) UMAP plot obtained from scRNA-seq analysis of Iso and SNCA 3x ENLs at day 70 after start of differentiation, showing clustering of each cell line after batch effect correction. (D) UMAP plot obtained from scRNA-seq analysis of Iso and SNCA 3x ENLs at day 70 after start of differentiation, showing clusters identified after annotation. (E) Ridgeplot showing the expression of SNCA per condition considering each cluster identified. n=3 biological replicates per group, p values calculated by unpaired two-tailed Student’s t test. (F) Heatmap comparing the cellular communication between SNCA 3x and Iso ENLs in total cells, with the top color bar representing the sum of the column values displayed in incoming signals and the right color bar representing the sum of outgoing signals, red or blue indicating increased or decreased signal of SNCA 3x compared with Iso, respectively. Data was generated using CellChat. (G) Barplots showing the quantification of the number of inferred interactions (top) and interaction strength (bottom) in iPSC-ENLs total cells. Data was generated using CellChat. (H) Differences in the overall signaling pathway between SNCA 3x and Iso ENLs in total cells, with the ranking indicating the importance of the pathways; red indicating the signaling pathways enriched in Iso, blue representing the signaling pathways enriched SNCA 3x, and black representing no difference in signaling pathway enrichment in groups.

Article Snippet: For intracellular staining of iPSCs, we used a combination of Nanog (cat: 130-120-704, Miltenyi) and Lin28A (cat:563597, BD Biosciences) primary antibodies (both 1:100) in BD Perm/Wash Buffer for 30 minutes, followed by washing and resuspension in 300μl FACS buffer.

Techniques: Flow Cytometry, Staining, Gene Expression, Marker, Derivative Assay, Quantitative RT-PCR, Expressing, Two Tailed Test, Generated, Protein-Protein interactions

Journal: bioRxiv

Article Title: Manipulation of the nucleoscaffold potentiates cellular reprogramming kinetics

doi: 10.1101/2023.03.12.532246

Figure Lengend Snippet:

Article Snippet: Nanog Antibody, anti-human, Vio B515, REAfinity, Clone REA314 , Miltenyi Biotec , 130-129-260.

Techniques: Recombinant, Saline, Membrane, Staining, Western Blot, Knock-Out, Microscopy, SYBR Green Assay, Transfection, Purification, Control, RNA Sequencing, Software